Anticancer Effect of Essential Oil of Seed of Ferula Assa-foetida on Adenocarcinoma Gastric Cell Line

Seyyed Majid Bagheri; Alia Shahmohamadi

doi:10.5530/ijcep.2020.7.3.24

Seyyed Majid Bagheri [1]Department Physiology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran [2] Hematology and Oncology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IRAN
Alia Shahmohamadi Department Physiology, International campus, Shahid Sadoughi University of Medical Sciences, Yazd, IRAN

DOI: https://doi.org/10.5530/ijcep.2020.7.3.24

Keywords: Ferula assa-foetida, Seed, Gastric cancer, Essential oil, Anticancer activity

Abstract

Background and Aim: Gastric cancer is a leading cause of cancer-associated mortality in men and the incidence is also on the rise in the entire world. The present study was aimed to investigate the chemical composition of essential oil of the seed of Ferula assafoetida (EOSF) and anticancer activity of the oil on adenocarcinoma gastric (AGS) cell line. Methods: For evaluation of the cytotoxicity effect of EOSF, AGS cells were exposed to EOSF at different concentration and for different time durations. The 3-(4, 5-dimethylthiazol- 2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay were carried out to characterize the cytotoxicity of the EOSF on AGS cells. In this study, the EOSF was obtained and analyzed by gas chromatography–mass spectrometry (GC-MS). Results: Our MTT assay showed that EOSF has a significant cytotoxicity activity in a time and concentration dependent manner. The main constituents of EOSF were alpha-D-Xylofuranoside, methyl 2, 5-di-O-methyl- (30.2%), E-1-propenyl sec-butyl disulfide (13.13%) Z-1-propenyl sec-butyl disulfide (11.34%). Conclusion: We conclude that the studied oil have a strong anticancer effect against AGS cell line. Based on the overall strong inhibition effects of the oil, isolation and studying of its compounds is suggested.