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 Table of Contents  
ORIGINAL ARTICLE
Year : 2017  |  Volume : 4  |  Issue : 4  |  Page : 170-173

Assessment of subclinical derangement of cardiovascular autonomic regulation in stable asthmatic patients


1 Department of Respiratory Medicine, Pt. B.D. Sharma, PGIMS, Rohtak, Haryana, India
2 Department of Physiology, Pt. B.D. Sharma, PGIMS, Rohtak, Haryana, India

Date of Web Publication29-Jan-2018

Correspondence Address:
Rajesh Gupta
H. No. 32/9J, Medical Campus, PGIMS, Rohtak - 124 001, Haryana
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijcep.ijcep_54_17

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  Abstract 

Background and Aim: Autonomic nervous system(ANS) is linked to asthma. The ANS controls several aspects of airway function. It was planned to assess the autonomic cardiovascular regulation in stable asthmatic patients by short-term heart rate variability(HRV) analysis. Methods: The present study was conducted in the Department of Respiratory Medicine in collaboration with the Department of Physiology, Pt. B.D.S PGIMS, Rohtak in a total of eighty subjects/patients with comparable age, sex, weight, and height. Two groups with forty subjects/patients in each were formed as follows: Group1: stable asthmatics and Group2: nonasthmatics(controls). Electrocardiography and HRV of the participants were measured using digitalized polyrite-D as per the standard protocol. Results: Frequency domain parameters showed statistically significant difference between two groups. Low frequency(LF)(nu) was(48.9±17.8vs. 53.3±16.9) significantly less(P<0.05), while high frequency(HF)(nu) significantly more(41.4±15.19vs. 38.3±1.2) in Group1 as compared to Group2. LF/HF (1.2±0.6vs. 1.39±0.78) ratio was decreased significantly(P<0.05) in Group1. Conclusion: An overall reduced HRV(sympathetic activity) and deranged sympathovagal balance with vagal dominance(decreased LF, increased HF) in stable asthmatics. Hence, HRV could serve as a potential noninvasive tool to assess autonomic cardiovascular regulation in asthmatics.

Keywords: Asthma, heart rate variability, sympathovagal


How to cite this article:
Gupta R, Gupta A, Kaushik NK, Chabbra R, Sood S. Assessment of subclinical derangement of cardiovascular autonomic regulation in stable asthmatic patients. Int J Clin Exp Physiol 2017;4:170-3

How to cite this URL:
Gupta R, Gupta A, Kaushik NK, Chabbra R, Sood S. Assessment of subclinical derangement of cardiovascular autonomic regulation in stable asthmatic patients. Int J Clin Exp Physiol [serial online] 2017 [cited 2018 Jul 22];4:170-3. Available from: http://www.ijcep.org/text.asp?2017/4/4/170/224169


  Introduction Top


Asthma is a chronic inflammatory condition of airway hyperresponsiveness that leads to recurrent episodes of wheezing, breathlessness, chest tightness, and coughing particularly at night or in the early morning. These episodes are associated with widespread but variable airway obstruction within lung that is often reversible either spontaneously or with treatment.[1]

Asthma is a major cause of disability, health resource utilization, and poor quality of life for those who are affected. It accounts for considerable health-care costs and loss of work productivity. Recently, it was estimated that 300 million people worldwide have asthma and projected that this number would increase to 400 million by 2025 as countries become more urbanized.[2]

There is no definite cause of asthma. There are host factors(genetics, obesity, and sex) and environmental factors(indoor allergens, air pollution, infections, tobacco smoke, etc.) that influence the risk of development of asthma.[3] Autonomic nervous system(ANS) is also linked to asthma. The ANS controls several aspects of airway function. Any abnormality in autonomic regulation of the airways may lead to bronchospasm, airway edema, and excessive mucous secretion, which are the events that place in pathogenesis of airway obstruction in bronchial asthma.[4]

The measurement of heart rate variability(HRV) is a relatively new powerful noninvasive methodology which can evaluate sympathovagal balance within the body. Impaired autonomic regulation of heart rate has been associated with various lung diseases such as asthma and chronic obstructive lung disease. Therefore, it was planned to assess the autonomic cardiovascular regulation in stable asthmatic patients by short-term HRV analysis.


  Materials and Methods Top


The present study was conducted in the Department of Tuberculosis(TB) and Respiratory Medicine in collaboration with the Department of Physiology, Pt. B.D.S PGIMS, Rohtak. The study design involved eighty individuals which were divided into two groups as follows:

  • Group1: Stable asthmatics(n=40)
  • Group2: Age-and sex-matched healthy controls(n=40).


Study participants were selected using the following inclusion and exclusion criteria.

Inclusion criteria

Stable asthmatic patients having

  • Symptom frequency<2 per week
  • Night time symptoms<2 per month
  • % forced expiratory volume in 1 s(FEV1) <80% of the predicted
  • %FEV1 variability of<20%
  • %FEV1 improvement of>12% following bronchodilator therapy.[5]


Exclusion criteria

  • Patients not willing to be assessed as per study protocol
  • Patients having acute exacerbation of asthma
  • Patients having any other cardiac or respiratory disease, hypertension, diabetes mellitus, and other diseases affecting ANS
  • Patients on drugs which are known to modify ANS, for example, beta blockers.


Spirometry was also done to measure the indices namely

  • FEV1
  • Forced vital capacity(FVC)
  • FEV1/FVC ratio.


This study included patients attending the Outpatient Department of TB and Respiratory Medicine.

Electrocardiography(ECG) and HRV of the subjects were measured with digitalized “POLYRITE D” as per the standards laid down by a task force of the European Society of Cardiology and the North American Society of Pacing and Electrophysiology.[6] Following settings were used:

  • Sensitivity–20 mv
  • Low-pass filter–2 KHz and high-pass filter–0.5Hz
  • Smoothening type–triangular(Bartlett) window
  • FFT(Fast Fourier transformation) size–1024(1K)
  • Frequency band used is 0.04


Procedure

After the preliminary history taking and examination, subjects were asked to lie down on the couch in front of the POLYRITE D system. The three disposable adhesive electrodes were attached to the left arm, right arm, and left leg, respectively, and for ECG measurement, lead 2 was selected. The basal recording of ECG(lead 2) was done for 5min.

The artifacts produced due to movement may cause a discrepancy in the result which is a major limitation in the interpretation of the same. We took utmost care to minimize the movements as the subjects were instructed not to move and not to speak while the recording is in progress to eliminate the artifacts.[7],[8] We carried out the recordings in supine position to avoid the influence of posture.[9]

ECG report was autogenerated by the machine. The heart rate is based on the duration of time interval between two R waves, graphically represented in the form of RR interval tachogram. The following variables were generated and used in the study.

Time domain parameters(in milliseconds) include:

  • SDNN–Standard deviation of the all NN interval
  • SDANN–Standard deviation of the average NN intervals calculated over short periods, usually 5min
  • rMSSD–Square root of the mean of the sum of the squares of differences between adjacent NN interval
  • NN50–Adjacent NN intervals that are the>50 ms
  • pNN50(%)–Percentage of difference between adjacent NN intervals that are greater than 50 ms.


Frequency domain indices include

  • TP(ms 2)–Total power
  • VLF(ms 2)–Very LF band variation
  • LF(AV) absolute values in ms 2 and in normal units(nu)–LF band variation
  • HF(AV) in ms 2 and in nu–HF band variation
  • LF/HF ratio


Subjects were explained the purpose of the study, the study protocol, and the informed consent was taken.

Statistical analysis

Statistical analysis was performed usingSPSS version17.0 software (SPSS Inc., Chicago, IL, USA). Mean and standard deviation of time and frequency domain HRV indices were measured, and significance of the difference between the two groups was tested by applying Student's t-test. Statistical significance was tested at 5% and expressed in terms of “P” value with P <0.05=statistically significant.


  Results Top


The study was carried out in the Department of Tuberculosis and Respiratory Medicine in collaboration with the Department of Physiology, Pt. B.D.S PGIMS, Rohtak. Two groups of study subjects were examined in the study. Group1 comprised of forty stable asthmatics and Group2 of forty age-and sex-matched healthy controls.

Anthropometric characteristics of the subjects are shown in [Figure1].
Figure1: Anthropometric characteristics of the study subjects

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Body mass index(BMI) of the Group1 differed significantly (P<0.01) from Group2, BMI being higher in Group1.

[Figure2] shows a comparative analysis of the spirometric indices, i.e.FEV1, FVC, peak expiratory flow rate(PEFR), and FEV1% between the two groups. The figure clearly illustrates the statistically significant(P<0.05) difference between the two groups in relation to the spirometric parameters which have been found to be low in Group1(stable asthmatics).
Figure2: Comparison of spirometric indices between Group1 and Group2

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[Figure3] compares the time domain parameters between the control group and Group1. All the time domain parameters were decreased in Group1, and the difference was statistically significant(P<0.05).
Figure3: Comparison of time domain parameters between Group1 and Group2

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[Figure 4] and [Figure 5] compare the frequency domain parameter between the two groups. All the frequency domain parameters showed statistically significant(P<0.05) difference between the two groups. TP, VLF, LF, and LF/HF ratio were decreased and HF increased in Group1 as compared to Group2.
Figure4: Comparison of frequency domain parameters between Group1 and Group2

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Figure5: Comparison of frequency domain parameters between Group1 and Group2

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  Discussion Top


Asthma is defined as the disease of the airways characterized by airway hyperresponsiveness, chest tightness, and wheeze.[1] Asthma is linked to ANS abnormalities. ANS controls several aspects of airway function such as airway tone and mucous secretion. Altered cardiovascular and respiratory responses reflecting autonomic abnormality are due to the common central origin of cardiovascular and respiratory autonomic efferent fibers. ANS abnormalities can be measured by measurement of HRV. HRV is a simple method to evaluate sympathovagal balance at the sinoatrial level. HRV represents continuous fluctuations in heart rate.

Time domain analysis of HRV uses statistical methods to quantify the variation of the standard deviation or the differences between successive R-R intervals. Frequency domain analysis of HRV enables us to calculate the respiratory-dependent HF and LF powers. HF power is mediated by vagal activity, while LF power has been suggested to represent both sympathetic and parasympathetic activity but predominantly sympathetic modulation. Whereas LF/HF ratio reflects global sympathovagal balance.[10]

Our study showed that time domain parameters were decreased significantly in Group1(stable asthmatics) as compared to Group2(controls), and this difference was statistically significant(P<0.05). Comparison of frequency domain parameters revealed that TP, VLF, LF, and LF/HF ratio were decreased and HF increased in Group1 as compared to Group2 and these changes were significant statistically(P<0.05). These results were comparable with the earlier HRV researches in asthmatic patients. Garrard et al. evaluated ten healthy controls, nine asymptomatic, untreated asthmatic subjects, and ten asthmatic patients during treatment of asthma, by measurement of variation in resting heart rate using frequency spectrum analysis. The study showed a significantly lower LF component in both asymptomatic and acute asthma subjects as compared to the controls. However, this study failed to prove the dominance of parasympathetic modulation(HF component) in asthmatic subjects.[11] In contrast, Du et al. were able to demonstrate enhanced vagal tone when comparing 23 healthy volunteers and 69 asthmatic young adults. In addition, the study showed diminished LF and SDANN in the test group.[12]

Kazuma et al. analyzed the 24h HRV in asthmatic children(ages 5–15years). These subjects were divided into groups according to the severity of their asthma. The autonomic nerve function(ANF) of asthma subjects was lower in comparison to the normal group. SDNN and LF were lowest in severe asthma group. Kazuma etal. also examined the circadian rhythm of nervous function in asthmatic children. Circadian rhythm disappeared in 11.25% of asthmatic children and was observed in all the healthy children. Moreover, the parasympathetic activity was low during periods of remission.[13] Ostrowska-Nawarycz et al. reported that, at resting conditions, a tendency for HF component of HRV to be higher with greater intensity of asthma. They observed that, in youth group with moderate asthma, the HF component at rest was significantly high.[14] A study by Gupta et al. showed significantly low VLF component of HRV in asthmatic group as compared to the control group.[15] Another study by Behera et al. correlated spirometric indices, namely, FEV1, PEFR, and FEV1/FVC with HRV parameters HF and LF in chronic smokers.[16] It is evidenced from these studies that higher level of asthma control is associated with improved sympathetic(LF Norm and LF/HF) and depressed parasympathetic(HF Norm) modulations.


  Conclusion Top


It can be deduced from the observations of our study that a probable compromised central sympathetic outflow and enhanced vagal tone in asthmatics lead to an imbalance in the sympathovagal interplay at the periphery and an increased propensity to airway obstruction, an essential feature of bronchial asthma. Hence, HRV could serve as a potential noninvasive tool in monitoring the patients of bronchial asthma. However, further elaboration and validation will be needed for HRV to become an effective diagnostic and prognostic clinical indicator in patients of bronchial asthma.

Acknowledgment

We would like to express our gratitude toward the laboratory technicians who helped us in performing the tests. We are also grateful to the subjects and the patients who took out their valuable time to participate in the study.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

 
  References Top

1.
MasoliM, FabianD, HoltS, Beasley R; Global Initiative for Asthma(GINA) Program. The global burden of asthma: Executive summary of the GINA dissemination committee report. Allergy 2004;59:469-78.  Back to cited text no. 1
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Global initiative for asthma guidelines. 2013. p.2-5. Available from: www.ginasthma.org. [Last accessed on 2017 Nov].  Back to cited text no. 2
    
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FantaCH. Asthma. NEngl J Med 2009;360:1002-14.  Back to cited text no. 3
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ThomasM, BrutonA, MoffatM, ClelandJ. Asthma and psychological dysfunction. Prim Care Respir J 2011;20:250-6.  Back to cited text no. 4
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GeorgeRB. Chest Medicine: Essentials of Pulmonary and Critical Care Medicine. 5thed. Philadelphia: Lippincott Williams and Wilkins; 2005. p.62.  Back to cited text no. 5
    
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MalikM, BiggerJT, CampAJ, KleigerRE, MaliniA, MossAJ, etal. Task Force of European Society of Cardiology and North American Society of Pacing and Electrophysiology. Heart rate variability standards of measurement, physiological interpretation and clinical use. Euro Heart J 1996;17:354-81.  Back to cited text no. 6
    
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SadchikovaMN, OrlovaAA. Clinical picture of the chronic effect of energy waves. Ind Hyg Occup Dis 1964;2:16-22.  Back to cited text no. 7
    
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BrennerKM, ThomasS, ShepherdRJ. Autonomic regulation of the circulation during exercise and heat exposure: Inferences from the heart rate variability. Sports Med 1998;26:85-99.  Back to cited text no. 8
    
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ParazziniM, RavazzaniP, TognolaG, Thuróczy G, MolnarFB, SacchettiniA, etal. Electromagnetic fields produced by GSM cellular phones and heart rate variability. Bioelectromagnetics 2007;28:122-9.  Back to cited text no. 9
    
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PomeranzB, MacaulayRJ, CaudillMA, KutzI, AdamD, GordonD, etal. Assessment of autonomic function in humans by heart rate spectral analysis. Am J Physiol 1985;248:H151-3.  Back to cited text no. 10
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GarrardCS, SeidlerA, McKibbenA, McAlpineLE, GordonD. Spectral analysis of heart rate variability in bronchial asthma. Clin Auton Res 1992;2:105-11.  Back to cited text no. 11
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DuJ, HeJ, WangY. Astudy of heart rate variability in asthma. Zhonghua Jie He He Hu Xi Za Zhi 2001;24:744-5.  Back to cited text no. 12
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KazumaN, OtsukaK, MatsuokaI, MurataM. Heart rate variability during 24 hours in asthmatic children. Chronobiol Int 1997;14:597-606.  Back to cited text no. 13
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Ostrowska-NawaryczL, Wroński W, Błaszczyk J, Buczyłko K, NawaryczT. The heart rate variability analysis in youth and children with bronchial asthma. Pol Merkur Lekarski 2006;20:399-403.  Back to cited text no. 14
    
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GuptaJ, DubeA, SinghV, GuptaRC. Spectral analysis of heart rate variability in bronchial asthma patients. Indian J Physiol Pharmacol 2012;56:330-6.  Back to cited text no. 15
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BeheraJK, SoodS, KumarN, SharmaK, MishraR, RoyPS, etal. Heart rate variability and its correlation with pulmonary function test of smokers. Heart Views 2013;14:22-5.  Back to cited text no. 16
[PUBMED]  [Full text]  


    Figures

  [Figure1], [Figure2], [Figure3], [Figure 4], [Figure 5]



 

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